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The stationary phase may be a strong or liquid over a stable assist. The mechanisms to blame for distribution among phases contain surface absorption, ion Trade, relative solubilities and steric affects289, 290, 291, 292. Substantial functionality liquid chromatography is often a valuable technique for quinolizidine alkaloid analysis, especially wh

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-hydroxybenzoic acid—on the nonpolar C18 column employing a aqueous cell stage that features a buffer of acetic acid and sodium acetate. Retention times are shorter for significantly less acidic cell phases simply because Every single solute is current within an anionic, weak foundation sort that is considerably less soluble during the nonpolar s

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